l 1 yeast nitrogen base (Thermo Fisher)

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Thermo Fisher l 1 yeast nitrogen base
L 1 Yeast Nitrogen Base, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l+1+yeast+nitrogen+base/YEAST+NITROGEN+BASE+WITHOUT+AM/pm41969221-216-14-26
Average 95 stars, based on 1 article reviews

l 1 yeast nitrogen base - by Bioz Stars, 2026-10

95/100 stars

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Modification:

Article Title: Thiol post-translational modifications modulate allosteric regulation of the OpcA-G6PDH complex through conformational gate control.
Article Snippet: .. The continuous light culture was grown in a modified BG-11 medium containing 0.09 g L 1 Yeast Nitrogen Base without amino acids and ammonium sulfate (H26271.36, Thermo Fisher Scientific Inc., USA), 0.264 g L 1 of (NH4)2SO4 (J64180.A1, Thermo Fisher Scientific Inc., USA), and 0.174 g L 1 of K2HPO4, (60,356, SigmaAldrich, USA). ..

Article Title: [2410.09346] Transcriptome and Redox Proteome Reveal Temporal Scales of Carbon Metabolism Regulation in Model Cyanobacteria Under Light Disturbance
Article Snippet: .. This microbe was cultured in a modified BG-11 medium 0.09 g L−1 Yeast Nitrogen Base without amino acids and ammonium sulfate (H26271.36, Thermo Fisher Scientific Inc., USA), 0.264 g L−1 of (NH4)2SO4 (J64180.A1, Thermo Fisher Scientific Inc., USA), 0.174 g L−1 of K2HPO4, (60,356, Sigma-Aldrich, USA), and 1 mM of isopropyl-β-d-thiogalactopyranoside (I56000, RPI, USA) at 30°C with a 1% CO2 supply. ..

Article Title: A toolkit for facilitating markerless integration of expression cassettes in Komagataella phaffii via CRISPR/Cas9.
Article Snippet: Geneticin (G418; Sigma-Aldrich) at 500 μg mL−1 was used for selection of CRISPi plasmids. .. The DM1-2 defined medium, used for kinetic growth assays, is a modification of the DM1 medium [74] containing 1.7 g L−1 Yeast Nitrogen Base, 7 g L−1 urea, 10 g L−1 KH2PO4 pH 6.5, 22 g L−1 D(+)-Glucose·H2O (all from Sigma-Aldrich), 1× Gibco MEM amino acids solution, 1× Gibco Cholesterol Lipid Concentrate (both from ThermoFisher Scientific) and 100 μL L−1 PTM1s trace mineral solution. .. PTM1s was prepared by dissolving 0.2 g L−1 biotin, 0.02 g L−1 boric acid, 0.92 g L−1 CoCl2·6H2O, 6 g L−1 CuSO4·5H2O, 65 g L−1 FeSO4·7H2O, 3 g L−1 MnSO4·H2O, 0.08 g L−1 NaI, 0.2 g L−1 Na2MoO4·2H2O, 20 g L−1 ZnCl2, 5 mL L−1 H2SO4 (all from Sigma-Aldrich) in milliQ-H2O and subsequent sterile filtration.

other:

Article Title: Spontaneously established syntrophic yeast communities improve bioproduction.
Article Snippet: OD of all five output plates was measured again at 48 h. Strains were grown in YPD (2% (wt/vol) glucose (Sigma-Aldrich, G8270), 20 g l−1 peptone (Bacto, 211677) and 10 g l−1 yeast extract (Bacto, 212750)); SM media (2% (wt/vol) glucose and 6.7 g l−1 yeast nitrogen base without amino acids (Sigma-Aldrich, Y1251)); SC-His (2% (wt/vol) glucose, 6.8 g l−1 yeast nitrogen base, 0.56 g l−1 CSM-His-Leu-Met-Trp-Ura (powder; MP Biomedicals), 60 mg l−1 leucine, 20 mg l−1 methionine, 40 mg l−1 tryptophan and 20 mg l−1 uracil) or SC-Ura (2% (wt/vol) glucose, 6.8 g l−1 yeast nitrogen base, 0.56 g l−1 CSM-His-Leu-Met-Trp-Ura (powder; MP Biomedicals, 114550422), 60 mg l−1 leucine (Thermo Nature Chemical Biology Article https://doi.org/10.1038/s41589-023-01341-2 Fisher Scientific, AC125125000), 20 mg l−1 methionine (Thermo Fisher Scientific, A1031836), 40 mg l−1 tryptophan (Thermo Fisher Scientific, 140591000) and 20 mg l−1 histidine (Thermo Fisher Scientific, 166155000)).

Cell Culture:

Article Title: Ammonium permease-based sensing mechanism for rapid ammonium activation of the protein kinase A pathway in yeast.
Article Snippet: .. For experiments with nitrogen-starved cells, the cells were first cultured at 30°C to exponential phase (OD600 = 1.5–2.0) in a defined minimal medium containing 1.7 g l−1 yeast nitrogen base without amino acids and without ammonium sulphate (Remel), 0.77 g l−1 complete supplement medium without uracil (CSM-URA; BIO 101) and 2% D-glucose. .. Exponential-phase cells were harvested, resuspended in nitrogen starvation medium (0.17% yeast nitrogen base without amino acids and ammonium sulphate; Remel) containing 4% glucose and incubated under shaking for 24 h at 30°C.

Passaging:

Article Title: Deep mutational learning for the selection of therapeutic antibodies resistant to the evolution of Omicron variants of SARS-CoV-2.
Article Snippet: Using 50 μg insert and 10 μg pYD1 backbone, electrocompetent EBY100 was transformed using 2 mm electroporation cuvettes (Sigma-Aldrich, Z706086). .. The cells were recovered for 1 h in recovery medium (YPD: 1 M sorbitol solution mixed in a 1:1 ratio) before passaging the cells into selective SD-CAA medium (20 g l−1 glucose (Sigma-Aldrich, G8270), 8.56 g l−1 NaH2PO4·H2O (Roth, K300.1), 6.77 g l −1 Na2HPO4·2H2O (Sigma-Aldrich, 1.06580), 6.7 g l −1 yeast nitrogen base without amino acids (Sigma-Aldrich, Y0626) and 5 g l−1 casamino acids (Gibco, 223120) in deionized water). ..

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